Studies on Hemagglutination and Hemagglutination Inhibition Test of Rubella Virus
نویسندگان
چکیده
منابع مشابه
An improved diluent for rubella hemagglutination and hemagglutination-inhibition tests.
Rubella hemagglutinating (HA) antigen was prepared in BHK-21 tissue as 5% cell suspensions and from unconcentrated and 20x concentrated infected supernatant fluids. In some instances, unconcentrated fluids were treated with Tween 80 and ether; cell suspensions were treated with ether alone. Preparations were tested for HA activity in dextrose-gelatin-Veronal (DGV) buffer solutions; 0.85% NaCl; ...
متن کاملAnalysis of the diluents used for rubella virus hemagglutination and hemagglutination-inhibition tests.
A new diluent (ADGP) for the rubella virus hemagglutination (HA) and hemagglutination-inhibition (HI) tests is described. It was found that the HA and HI titers and the erythrocyte agglutination pattern were improved in ADGP compared to previously described diluents. The influence of the components of ADGP and of various test conditions on optimal HA and HI results were examined.
متن کاملComparison of hemagglutination inhibition test and enzyme-linked immunosorbent assay for determining antibody to rubella virus.
The hemagglutination inhibition test (HAI) and the enzyme-linked immunosorbent assay (ELISA) for detecting antibody to rubella virus were compared by testing 25 sets of paired sera taken before and after infection and 10 sets of sera taken during acute and convalescent stages of the disease and by screening 700 serum samples from the Collaborative Perinatal Project, NIH/NINCDS. The tests were f...
متن کاملHepatitis A virus hemagglutination and a test for hemagglutination inhibition antibodies.
Like enteroviruses, hepatitis A virus (HAV) hemagglutinated various species of erythrocytes under similar conditions. HAV-specific antibodies in both acute- and convalescent-phase sera were found to inhibit hemagglutination. The HAV hemagglutination inhibition test can be used for diagnosis, epidemiological surveillance, and vaccine assessment.
متن کاملSpecificity of the passive hemagglutination test for antibody to rubella virus and the passive hemagglutination response after vaccination.
Nonspecific reactions by the passive hemagglutination test for rubella viral antibody occurred with 1.5% of sera tested. Rises in passive hemagglutination titers (greater than or equal to 4X) occurred with some serum samples taken 14 days after vaccination.
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 1970
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.44.165